Alex Powell Chemistry Lab 4/5/2012 Crime research laboratory I. In this raveling ground you are finding the parsimoniousness of your inglorious which isnt a real blood serum. You are using a unmistakable spectroscopy which is the measurement of the numerate of light absorbed by a solution. My hypothesis is that this method is valid and reliable. II. Some materials employ in this lab were spectrophotometer cuvettes, test underground rack, pipet, veriner and a spectrophotometer. third. world-class thing you do is you center seven spectrophotometer cuvettes in a small test tube rack. Make sure you represent along the rigid location because fingerprints on the clear sides bottom mess up the readings. You then pipet the amounts of material listed in Table 1 of your lab manual, then shake the minglees thoroughly. numeral on you spectrophotometer and set the wavelength telephone dial 540 nm. Allow the instrument to tippy up for ten minutes. wherefore prepare th e unknown warning by pipetting 1 ml of the unknown solution into the cuvette and mix with 1 ml of spare back ferric process. Then after(prenominal) that you calculate the parsimoniousness for from each one of the solutions. Cuvette #| 25 mg/dl Standard| H2O ml| Dilute Iron III Nitrate (ml)| .039 M HNO3 (ml)| concentration| 1| 0| 1| 0| 1| 0| 2| 0.1| 0.9| 1| 0| 0.09| 3| 0.3| 0.7| 1| 0| 0.287| 4| 0.5| 0.5| 1| 0| 0.
407| 5| 0.7| 0.3| 1| 0| 0.706| 6| 1| 0| 1| 0| 1.316| When then use the calibration skid to determine the concentration of the unknown and we get .747. IV. As you basin see from o ur data as the amount of H2O decreases and t! he 25mg/dl tired increases as does our concentration for the salicylic acids. We keep the amount of dilute iron III nitrate the same because its our freelancer inconsistent. With the unknowns concentration we know it falls mingled with .7mbg/dl of standard and only .3 ml of water. My guess is that its just high the .7 because the concentration is just a teensy bit higher(prenominal) so maybe .71 or .72. The dependent variable in this lab is the...If you want to get a to the full essay, do it on our website: BestEssayCheap.com
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